Fig. 2: Cryo-EM structure of the R2Tg retrotransposon initiating TPRT. | Nature Communications

Fig. 2: Cryo-EM structure of the R2Tg retrotransposon initiating TPRT.

From: Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons

Fig. 2: Cryo-EM structure of the R2Tg retrotransposon initiating TPRT.The alternative text for this image may have been generated using AI.

A Cartoon of the R2Tg cryo-EM structure. Stars represent active sites. B Atomic model for the R2Tg TPRT complex. The Myb domain is flexibly attached, indicated by dotted outlines. ZnF, zinc finger; RT, reverse transcriptase; RLE, restriction-like endonuclease. C Corresponding view of the previously determined structure of the R2Bm TPRT complex19. D Structure of the ordered core of the R2Tg 3′ UTR and the associated RT − 1 domain. E Secondary structure diagram of the R2Tg 3′ UTR compared to the corresponding core of the R2Bm 3′ UTR. Non-canonical base pairs are shown according to the notation of Leontis and Westhof96. F 28S rDNA binding by R2Bm compared with R2Tg. Left: bases shown in black form hydrogen bonds to the R2 protein, which may indicate specific recognition of these bases. Right: purple lines indicate the hydrogen bonds in more detail.

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