Table 3 MS/MS analyses of HUWE1FL-driven compound ubiquitination

From: Selective ubiquitination of drug-like small molecules by the ubiquitin ligase HUWE1

compound

BI8622

BI8626

1

BI8622

BI8626

1

ATP

-

-

-

+

+

+

BI8622-Ub

X

X

X

yes

X

X

BI8626-Ub

X

X

X

X

yes

X

1-Ub

X

X

X

X

X

yes

  1. Table summarizing the results of targeted MS/MS analyses of reconstituted HUWE1FL-driven ubiquitination reactions, containing the indicated compounds ± ATP. 'yes' indicates detection of compound-modified tryptic C-terminal ubiquitin peptides characteristic of the conjugated products listed in the left column. For the corresponding assigned spectra, see Supplementary Figs. 15–17.
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