Table 2 Key resources table

From: Cellular and genetic mechanisms that shape the development and evolution of tail vertebral proportion in mice and jerboas

Reagent/Resource

Source

Identifier

Chemicals, peptides, etc

Alcian 8GX certified by Biological Stain Commission

Sigma-Aldrich

Cat# A3157

Alizarin red S

Sigma-Aldrich

Cat# A5533

OCT compound

Tissue-Tek

Ref# 4583

Proteinase K

Qiagen

Cat# 19131

MyTaq Red PCR mix

Bioline

Cat# BIO-25047

Calcein

Sigma-Aldrich

Cat# C0875

Eosin-Y

Thermo scientific

Cat# 201931000

Hematoxylin

Sigma-Aldrich

Cat# GHS132

CryoJane tape-transfer system

Leica

 

 Tape windows

Leica

Cat# 39475214

 Solution A

Leica

Cat# 39475270

 Solution B

Leica

Cat# 39475272

Fluoromount-G mounting medium

Invitrogen

Cat# 00-4958-02

Bioworld epitope unmasking buffer

Fisher scientific

Cat# 50-199-077

NPR3 anti-Rabbit polyclonal antibody

Invitrogen

Cat# PA5-96947

Alexa Fluor 594 Goat Anti-Rabbit IgG (H&L)

Invitrogen

Cat# ab150080

DAPI

Invitrogen

Cat# D1306

Critical commercial assays

Click-iTTM EdU Kit, Alexa Fluor 647

Invitrogen

Cat# C10340

RNeasy Micro Kit

Qiagen

Cat# 74004

QIAshredder

Qiagen

Cat# 79656

Experimental models: Organisms/strains

Mus musculus (CD-1 Strain)

Charles River Labs

Strain Code: 022

Mus musculus (C57BL/6 Strain)

Charles River Labs

Strain Code: 027

Mus musculus Npr3−/−

Olgin Lab, UCSF

MGI:2158355

Jaculus jaculus

UCSD Cooper Lab

 

Oligonucleotides

NPR3 primer Forward

3′ CACAAGGACACGGAATACTC 53′

NPR3 primer Reverse

3′ CTTGGATGTAGCGCACTATGTC 53′

NPR3 primer Neo Forward

3′ ACGCGTCACCTTAATATGCG 53′

Software and algorithms

FIJI97

https://imagej.net/software/fiji/

InteredgeDistance Macro for FIJI

Santosh Patnaik

DataViewer

Bruker

DESeq245

https://github.com/thelovelab/DESeq2

STAR100

https://github.com/alexdobin/STAR

TOGA44

https://github.com/hillerlab/TOGA

CutAdapt98

https://github.com/marcelm/cutadapt

FastQC99

https://github.com/s-andrews/FastQC

Trim Galore

https://github.com/FelixKrueger/TrimGalore

R packages used in analysis

 ggplot2

https://github.com/tidyverse/ggplot2

 dplyr

https://github.com/tidyverse/dplyr

 Plotly

https://github.com/plotly

 clusterProfiler47

https://github.com/YuLab-SMU/clusterProfiler

 Hrbrthemes version 0.8.7

https://github.com/hrbrmstr/hrbrthemes

 Paletteer version 1.3.0

https://github.com/EmilHvitfeldt/paletteer

 BiocManager version 1.30.25

https://github.com/Bioconductor/BiocManager

 GeneOverlap101 version 1.40.0

https://github.com/shenlab-sinai/GeneOverlap

 gprofiler2 version 0.2.3

https://github.com/egonw/r-gprofiler2

 Tidyr version 1.3.1

https://github.com/tidyverse/tidyr

 RColorBrewer version 1.1-3

https://github.com/cran/RColorBrewer

 Viridis version 0.6.5

https://github.com/sjmgarnier/viridis

 Reshape2

https://github.com/hadley/reshape

Graphpad Prism software

Adobe Creative Cloud (Illustrator and Photoshop)