Fig. 2: The aggregation of TAF-12, UAD-2, and the USTC complex at piRNA foci is interdependent. | Nature Communications

Fig. 2: The aggregation of TAF-12, UAD-2, and the USTC complex at piRNA foci is interdependent.

From: The H3K27me3 reader UAD-2 recruits a TAF-12-containing transcription machinery to initiate piRNA expression within heterochromatic clusters

Fig. 2: The aggregation of TAF-12, UAD-2, and the USTC complex at piRNA foci is interdependent.The alternative text for this image may have been generated using AI.

a Pachytene germ cells from animals expressing UAD-2::GFP and TAF-12::tagRFP. TAF-12 aggregates in piRNA foci and colocalizes with UAD-2. n = 6 independently imaged worms over three independent experiments with similar results. b, c Pachytene germ cells from animals expressing TAF-12::GFP and tagRFP::SYP-1 in the indicated genetic backgrounds. SYP-1 is a component of the synaptonemal complex and is used as a marker to label the chromosomes. n = 6 independently imaged worms over three independent experiments with similar results. d Fluorescence images of representative pachytene germ cells of the indicated animals expressing TOFU-4::GFP, SNPC-4::GFP or PRDE-1::GFP and TAF-12::AID (with or without auxin treatment). Scale bar, 5 μm. n = 4 independently imaged worms over three independent experiments with similar results.

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