Table 3 Deconvolution settings for each protein or protein complex used in this study
From: A practical method for determining the rate of covalent modification of fragments and leads
| Â | KRASG12C | SKP1-FBXW7 | SOCS2-EloB-EloC |
|---|---|---|---|
Mass range (kDa) | 10–60 | 15–55 | 8–35 |
Mass step (Da) | 1 | 1 | 1 |
m/z range | 800–1600 | 750–3000 | 700–3000 |
Time window (minutes) | 0.23–0.44 | 0.215-0.3 | 0.23-0.4 |
Peak signal-to-noise | ≥ 30 | ≥ 30 | ≥ 30 |
Maximum peak limit by height to the largest | 100 | 100 | 100 |
Minimum consecutive charge state | 5 | 5 | 5 |
Minimum protein fit score | 8 | 8 | 8 |
