Fig. 2: Ccdc78 is required for localization of distal tip proteins in MCCs. | Nature Communications

Fig. 2: Ccdc78 is required for localization of distal tip proteins in MCCs.

From: A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function

Fig. 2: Ccdc78 is required for localization of distal tip proteins in MCCs.The alternative text for this image may have been generated using AI.

A–C Image of GFP-Spef1 (green) and membrane-RFP (magenta) in Xenopus MCCs in control A, Ccdc78 MO-injected B or rescued embryos C with magnified view of cilium on the right. Scale bars represent 10 μm. D Schematic cartoon of Spef1 distribution in control and Ccdc78 MO-injected cilium. Quantification of fluorescent intensity of GFP-Spef1 along the axoneme was normalized by average intensity in control, Ccdc78 MO injected and rescued embryos. (n = 154 cilia, 45 cells, 9 embryos, 3 experiments) Data are presented as mean values +/- SD.E–H Localization of distal proteins in control (E, F) and Ccdc78 MO injected (G, H) embryos with magnified view and schematic cartoon with quantification of normalized mean intensity on the right. Embryos were injected with Membrane-RFP (magenta) and GFP-Cep104 (green, arrowhead and arrow). Scale bars represent 10 μm. (n = 80 cilia, 20 cells, 7 embryos, 3 experiments) Data are presented as mean values +/- SD. I–L Localization of distal proteins in control (I, J) and Ccdc78 MO injected (K, L) embryos with magnified view and schematic cartoon with quantification of normalized mean intensity on the right. Embryos were injected with Membrane-RFP (magenta) and GFP-Armc9 (green, arrow). Scale bars represent 10 μm. (n = 80 cilia, 20 cells, 7 embryos, 3 experiments) Data are presented as mean values +/- SD.

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