Fig. 4: Ccdc33 localizes to the extreme distal tip of MCC cilia interacting with Ccdc78 at the MCC cilia tip. | Nature Communications

Fig. 4: Ccdc33 localizes to the extreme distal tip of MCC cilia interacting with Ccdc78 at the MCC cilia tip.

From: A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function

Fig. 4: Ccdc33 localizes to the extreme distal tip of MCC cilia interacting with Ccdc78 at the MCC cilia tip.The alternative text for this image may have been generated using AI.

A–A”’ Localization of GFP-Ccdc33 (green) with RFP-membrane (magenta) during ciliogenesis on Xenopus embryo epithelium. Magnified view of cilium on the bottom right. Scale bar represents 10 μm. B Human tracheal epithelial cell multi-cilia stained with anti-acetylated tubulin (magenta) and anti-CCDC33 (green) antibody. Scale bar represents 1 μm. C–F Xenopus MCC was injected with membrane-RFP (magenta) and GFP-Ccdc33 (green, arrowhead) in control (C, D) and Ccdc78 KD (E, F) embryo with magnified view and schematic cartoon with quantification of normalized mean intensity on the right. Scale bar represents 10 μm. (n = 78 cilia, 30 cells, 10 embryos, 3 experiments) Data are presented as mean values +/- SD. G, H Xenopus MCC injected with membrane-RFP (magenta) and GFP-Ccdc78 (green, arrowhead) in control (G, H) and Ccdc33 MO-injected (I, J) embryo with magnified view and schematic cartoon with quantification of normalized mean intensity on the right. Scale bar represents 10 μm. (n = 78 cilia, 30 cells, 10 embryos, 3 experiments) Data are presented as mean values +/- SD.

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