Fig. 5: Ccdc33 is required for localization of distal tip proteins in MCC. | Nature Communications

Fig. 5: Ccdc33 is required for localization of distal tip proteins in MCC.

From: A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function

Fig. 5: Ccdc33 is required for localization of distal tip proteins in MCC.The alternative text for this image may have been generated using AI.

A–C Image of GFP-Spef1 (green) and membrane-RFP (magenta) injected Xenopus multi-cilia in control A, Ccdc33 MO-injected B, and rescued C embryos. Scale bars represent 10 μm. D Schematic cartoon of Spef1 distribution in control and Ccdc33 MO-injected cilium. Quantification of fluorescent intensity of GFP-Spef1 along the axoneme normalized by average intensity in control, Ccdc33 MO-injected and rescued embryos. (n = 65 cilia) Data are presented as mean values +/- SD. E–H Localization of distal proteins in control (E, F) and Ccdc33 MO-injected (G, H) embryos, with magnified view and schematic cartoon with quantification of normalized mean intensity on the right. Embryos were injected with membrane-RFP (magenta) and GFP-Cep104 (green, arrowhead and arrow). Scale bars represent 10 μm. (n = 180 cilia, 36 cells, 12 embryos, 3 experiments) Data are presented as mean values +/- SD. I–L Localization of distal proteins in control (I, J) and Ccdc33 MO-injected (K, L) embryo with magnified view and schematic cartoon with quantification of normalized mean intensity on the right. Embryos were injected with membrane-RFP (magenta) and GFP-Armc9 (green, arrow). Scale bars represent 10 μm. (n = 85 cilia, 27 cells, 9 embryos, 3 experiments) Data are presented as mean values +/- SD.

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