Fig. 6: Ccdc78 and Ccdc33 display microtubule bundling activity in vivo and in vitro. | Nature Communications

Fig. 6: Ccdc78 and Ccdc33 display microtubule bundling activity in vivo and in vitro.

From: A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function

Fig. 6: Ccdc78 and Ccdc33 display microtubule bundling activity in vivo and in vitro.The alternative text for this image may have been generated using AI.

A Workflow for generating mosaic expression in Xenopus embryo. B, C Image of Xenopus embryo goblet cells on epithelium expressing GFP-EMTB (green), membrane-BFP (blue), and membrane-RFP(magenta) in embryos injected with Myc-Ccdc78 600 pg (B–B”) and Myc-Ccdc33 600 pg (C–C”). Cells expressing membrane-RFP correspond to those overexpressing Myc-ccdc78 or Myc-ccdc33, indicating mosaic expression. Scale bars represent 10 μm. D–F Quantification of local thickness (n = 26, 13 embryos across 3 experiments). The distribution of local thickness was compared between control D and Ccdc78 600 pg E or Ccdc33 600 pg F using two-sided Kolmogorov-Smirnov test (KS-test), the KS-distance and P-value are shown on right. CTL vs Ccdc78 600 pg ****p < 0.0001, CTL vs Ccdc33 600 pg ***p = 0.0002. Data are presented as mean values +/- SD. G–J Image of 647-fluorophore labeled microtubules (gray) incubated with GFP G, GFP-Ccdc78 30 nM H, GFP-Ccdc33 20 nM I and GFP-Ccdc78 15 nM with GFP-Ccdc33 10 nM J. Scale bar represents 10 μm. G’–J’ Quantification of local thickness(n = 15 across 2 experiments). The distribution of local thickness was compared between control (G’) and Ccdc78 30 nM (H’) or Ccdc33 10 nM (I’) or GFP-Ccdc78 15 nM with GFP-Ccdc33 10 nM (J’) using two-sided Kolmogorov-Smirnov test (KS-test), the KS-distance and P-value are shown in right. GFP vs GFP-Ccdc78 30 nM **p = 0.0024, CTL vs GFP-Ccdc33 20 nM **p = 0.0024, GFP vs GFP-Ccdc78 15 nM & GFP-Ccdc33 10 nM ***p = 0.0007. Data are presented as mean values +/- SD.

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