Fig. 8: Deficiency of Ccdc33 shows heterogeneous MCC cilia phenotype. | Nature Communications

Fig. 8: Deficiency of Ccdc33 shows heterogeneous MCC cilia phenotype.

From: A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function

Fig. 8: Deficiency of Ccdc33 shows heterogeneous MCC cilia phenotype.The alternative text for this image may have been generated using AI.

A–C Confocal image of Xenopus MCC injected with membrane-RFP (magenta) and GFP-Ccdc78 (green) in Ccdc33 KD embryos. The magnified view of relatively longer B or shorter C cilium are shown on the right. Scale bar represents 10 μm. D Correlation between cilia length and GFP-Ccdc78 intensity at the ciliary tip in Ccdc33 KD embryos MCCs. Each dot represents a single cilium. A moderate positive correlation was observed (Pearson’s correlation coefficient, r = 0.5070). E–G Confocal image of Xenopus MCC injected with membrane-BFP (magenta) and Spef1-RFP (green) in Ccdc33 KD embryos. The magnified view of relatively longer F or shorter G cilium are shown on the right. Scale bar represents 10 μm. (n = 150 cilia, 37 cells, 13 embryos across 3 experiments). H Correlation between cilia length and Spef1-RFP intensity at the ciliary tip in Ccdc33 KD embryos MCCs. Each dot represents a single cilium. A moderate positive correlation was observed (Pearson’s correlation coefficient, r = 0.6672). (n = 123 cilia, 32 cells, 11 embryos across 3 experiments). I Confocal image of GFP-Spef1 and membrane-RFP labeled in control MCC with magnified view of cilia on the right. Scale bar represents 10 μm. J Confocal image of GFP-Spef1 and membrane-RFP labeled in MCC overexpressed with 700 pg of ccdc78 mRNA, magnified view of cilia on the right. Scale bar represents 10 μm. K Quantification of the length of cilium comparing between control and Ccdc78-overexpressed MCC. p = 0.6268 (n = 68 cilia, 20 cells, 9 embryos across 3 experiments) (Unpaired t-test) Data are presented as mean values +/- SD. (L-O) Image of Xenopus MCC labeled with GFP-Spef1 and membrane-RFP in control L, Ccdc33 Knockdown M, rescue with 600 pg of ccdc33 mRNA N and rescue with 600 pg of ccdc78 mRNA O, with magnified view of cilium on the right. Scale bar represents 10 μm.

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