Fig. 9: The function of C2 domain of Ccdc33 in organizing distal ciliary structure in MCC cilia. | Nature Communications

Fig. 9: The function of C2 domain of Ccdc33 in organizing distal ciliary structure in MCC cilia.

From: A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function

Fig. 9: The function of C2 domain of Ccdc33 in organizing distal ciliary structure in MCC cilia.The alternative text for this image may have been generated using AI.

A Alphafold prediction of Xenopus Ccdc33 structure. C2 domain is colored in blue and coiled-coil domain in gray. B Secondary structure of Xenopus Ccdc33 and truncation of C2 domain in Ccdc33. C2 domain is colored in blue and coiled-coil domain in gray. (C-E) Confocal image of Xenopus MCC injected with GFP-Spef1 and membrane-RFP in control C, Ccdc33 knockdown D and rescue with 600 pg of ccdc33-ΔC2 mRNA, with magnified view of cilium on the right. Scale bar represents 10 μm. F Quantification of the length of cilium. ****p < 0.0001 (n = 120 cilia, 40 cells, 10 embryos across 3 experiments) (Ordinary one-way ANOVA) Data are presented as mean values +/- SD. G Quantification of fluorescent intensity of GFP-Spef1 along the axoneme was normalized by average intensity in control, Ccdc33 MO injected and rescued with Ccdc33-△C2 embryos. (n = 120 cilia, 40 cells, 10 embryos, 3 experiments) Data are presented as mean values +/- SD.

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