Fig. 10: Rescue of embryonic lethality at elevated temperature through overexpressions, and clinal genes as “natural” candidates.
From: Elevated temperature fatally disrupts nuclear divisions in the early Drosophila embryo
A–C Testing the rescue of embryonic lethality and the fraction of mitotic failures with overexpression of αCat or sgg. A Scatter plot shows distribution of percent lethality in embryos from various genotypes, at normal (25 °C) vs elevated temperature (29 °C), calculated based on the fraction of embryos that don’t hatch into larvae. n = 10 (25 °C control), 11 (29 °C control), 10 (25 °C αCat-OE), 12 (29 °C αCat-OE), 8 (25 °C sgg-OE), 7 (29 °C sgg-OE) samples; each sample has ≥18 embryos. B, C Scatter plots show distribution of the fraction of mitotic failures in crowded regions (B), and in combined regions (C), in the embryos at normal (22 °C) vs elevated temperature (29 °C). n = 8 (22 °C control), 9 (29 °C control), 8 (22 °C αCat-OE), 8 (29 °C αCat-OE), 7 (22 °C sgg-OE), 7 (29 °C sgg-OE) embryos. D, E Analysis of “natural” candidate genes that vary along thermoclines. D Venn diagram illustrates how we constructed a non-redundant list of genes associated with “spindle dynamics” through the union of genes associated with GO terms, cell polarity (203 genes), spindle localization (37 genes), and spindle organization (138 genes). E Pie charts show clinal distribution scores for various genes associated with “spindle dynamics” as constructed in D (320 genes), or with GO term “metabolic process” (6612 genes). ~1% of genes associated with “spindle dynamics”, while ~0.2% of the genes associated with “metabolic process”, are clinal in all three continental datasets. See also, Supplementary Data 1 and 2. A–C shows scatters with median, with whiskers showing 95% CI. In B and C, large hollow markers, embryos with blastoderm holes; small hollow markers, embryos with small gaps in the blastoderm. Non-parametric Kruskal–Wallis test with Dunn’s uncorrected test for multiple comparisons: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; non-significant comparisons are not shown. Source data and exact p-values for plots are available in the Source Data file.
