Extended Data Fig. 2: The pattern of the whole-genome sequencing data covering the donor site of the 12,586-bp insertion (the insert donor region).
From: A HAK family Na+ transporter confers natural variation of salt tolerance in maize
a, Integrated Genome Viewer visualization of the whole-genome sequencing data covering the insert donor region. b, High resolution view of the left and right borders of the insert donor region. The dash line coupled the paired reads. We generated the resequencing library using uniform length DNA fragment of ~500 bp, therefore the average distance between two paired reads is ~500 bp. However, we found that the reads mapped to the left border of the insert donor region paired with the reads mapped to the right border of the insert donor (>12 kb away), indicating the sequence between the left and right border of the insert donor region has been deleted, thus indicating that the 12,586- bp insertion observed in ZmHAK4 of X178 is a translocation.
