Extended Data Fig. 6: HPLC-HR-MS analysis of cell wall esterified decarboxylated diFA from 3-[2H3]-P2, 6-[2H3]-P2, [2H3]-FA treatment groups and control.
From: A sucrose ferulate cycle linchpin for feruloylation of arabinoxylans in plant commelinids
![Extended Data Fig. 6: HPLC-HR-MS analysis of cell wall esterified decarboxylated diFA from 3-[2H3]-P2, 6-[2H3]-P2, [2H3]-FA treatment groups and control.](http://media.springernature.com/full/springer-static/esm/art%3A10.1038%2Fs41477-024-01781-1/MediaObjects/41477_2024_1781_Fig11_ESM.jpg?as=webp)
a, EIC of decarboxylated diFA (341.1020, C19H17O6, [M-H]-). a-h: refer to EIC of decarboxylated diFA. b, EIC of single FA-labeled decarboxylated diFA ([2H3]-decarboxylated diFA) (344.1208, C19H14D3O6, [M-H]-). c, EIC of double FA-labeled decarboxylated diFA ([2H6]-decarboxylated diFA) (347.1396, C19H11D6O6, [M-H]-). 1: control; 2 to 5: 13.9 μM 3-[2H3]-P2, 13.9 μM 6-[2H3]-P2, 13.9 μM [2H3]-FA and 27.8 μM [2H3]-FA treatment groups. a-h: EIC from treatment groups compared to control. Ion chromatogram marked red is selected to show the observed mass spectra of [2H3]-decarboxylated diFA and [2H6]-decarboxylated diFA from the control and the treatment groups (d). d, Mass spectra of decarboxylated diFA (Calcd. of C19H17O6 is 341.1020, [M-H]-), [2H3]-decarboxylated diFA (Calcd. of C19H14D3O6 is 344.1028, [M-H]-), [2H6]-decarboxylated diFA (Calcd. of C19H11D6O6 is 347.1396, [M-H]-) from control and 13.9 μM 3-[2H3]-P2, 13.9 μM 6-[2H3]-P2, 13.9 μM [2H3]-FA and 27.8 μM [2H3]-FA treatment groups.
