Fig. 1: Erythroblasts generated from PSCs and their differentiation in defined culture conditions after addition of ferric citrate.

a Schema for generating erythroblasts from PSCs via CD34 sorted HE. b After erythrocyte generation, morphological changes indicate that HE developed into erythroid lineage cells via erythroblasts. Expanded erythroblasts were continuously proliferated by ferric citrate. Definitive hematopoiesis involving megakaryocyte erythrocyte progenitors was observed, and matured erythroblasts were generated till day 32. To obtain mature erythroid lineage cells, OP9 stromal cells were co-cultured, and enucleated erythrocytes were detected on OP9 cells.