Extended Data Fig. 1: Host ATF4 deletion causes a transient weight loss and gender-independent increase in survival of B16F10 cells-injected mice.
a, Genotyping of mice for Rosa26::CreERT2 and ATF4 status. b, Box and whisker plot display the RT-qPCR of Atf4 in spleen and liver (n = 3–5 biologically independent samples per group). Unpaired two-sample t-test. c, Schema for injection of tumour cells post tamoxifen treatment. d, Tumour growth curves of single mouse plotted from B16F10 cells-injected mice. e, Kaplan-Meier survival analysis by gender from d. Log-rank (Mantel-Cox) test. f, Tumour growth curves of single mouse plotted from MH6419 cells-injected mice. g. Kaplan-Meier survival analysis of the mice following 5×105 MH6419 cell injection. Log-rank (Mantel-Cox) test. h, Box and whisker plot display % normal pancreas weight normalized to BW. i, Representative images of pancreas from orthotopic pancreatic tumour model, stained for H&E. The yellow and blue dotted lines indicate the tumour and normal areas of pancreas, respectively. j, Box and whisker plot of the % tumour area normalized to total area. Unpaired two-sample t-test.
