Extended Data Fig. 3: Assignment of His tautomers in ZF5.3 at pH 5.5. | Nature Chemistry

Extended Data Fig. 3: Assignment of His tautomers in ZF5.3 at pH 5.5.

From: The biophysical requirements that govern the efficient endosomal escape of designed mini-proteins

Extended Data Fig. 3: Assignment of His tautomers in ZF5.3 at pH 5.5.The alternative text for this image may have been generated using AI.

a, Results of hbCBcgcdHD experiments that provide the 13Cα and 13Cꞵ chemical shifts of each of the three His residues in ZF5.3, His15, His19, and His23. b, Results of a 2D CBHD experiment that correlates the chemical shift of each 13Cꞵ with the corresponding 1Hδ. c, 13C-CT HSQC provides the chemical shift of 13Cδ and 1Hδ. If the chemical shift of 13Cδ is less than 122 ppm the His residue is said to be in the α-tautomeric state and if the chemical shift of 13Cδ is greater than 122 ppm it is in the ꞵ-tautomeric state. d, Assignment of His15 as the α-tautomer and Zn(II) coordinating His19 and His23 as the ꞵ-tautomer.

Back to article page