Extended Data Fig. 3: Comparative transcriptome analysis of the two leaf color morphs of Corydalis hemidicentra.

a, PCA score plot of transcript profiles. b, Scatter plot of transcriptome levels. Fold change ≥ 2 and false discovery rate (FDR) ≤ 0.05 indicate significant difference of differentially expressed genes (DEGs). c, The expression pattern of DEGs. d, KEGG enrichment of DEGs. Enrichment P values are from Fisher’s exact test. The number of DEGs associated with each category is indicated in brackets after the term description, and enrichment values are indicated by color, as shown in the color scale. e, Expression levels of 12 anthocyanin genes in the two morphs. PAL phenylalanine ammonia-lyase, C4H cinnamate-4-hydroxylase, 4CL 4-coumarate CoA ligase 4, CHS chalcone synthase, CHI chalcone isomerase, F3H flavanone 3-hydroxylase, F3′H flavonoid 3′-hydroxylase, F3′5′H flavonoid 3′,5′-hydroxylase, DFR dihydroflavonol 4-reductase, ANS anthocyanidin synthase, UFGT flavonoid-3-O-glucosyltransferase, FLS flavonol synthase, FG2 flavonol-3-O-glucoside L-rhamnosyltransferase. Data represent the mean ± SD from four replicates. P values are from two-sided Student’s t test.