Extended Data Fig. 5: GC B Cells.
From: Bacterial and host fucosylation maintain IgA homeostasis to limit intestinal inflammation in mice
Gut Bacterial Fucosylation Modulates Activation of GC B Cells and Induction of Specific IgA Production in PPs. (a) Representative plots of flow cytometry and the frequency of follicular T cells (n = 6), regulatory B cells (n = 5) and dendritic cells (n = 4) in PPs of WT and Fut2-/- mice. Independent biological samples. (b) FISH analysis of microbiota localization in the colon using a universal bacterial 16S rRNA gene probe (Red). (c) Representative distal colons of WT (Top) and Fut2-/- (Bottom) mice stained with PAS-AB. (d) Representative plots of flow cytometry and the frequency of activated B220+ GL-7+IgD− GC B cells in PPs of WT and Fut2-/- mice at the age of 3-4 weeks and 20-24 weeks. (e) Representative plots of flow cytometry and the frequency of activated B220+ GL-7+IgD− GC B cells and CD138+IgA+ PCs in PPs of WT and Fut2-/- mice with antibiotic treatment for depletion of gut microbiota. (f-g) Representative ELISPOT and quantification of B. fragilis 9343-specific IgA secreting plasma cells and total IgA secreting plasma cells in PPs (f) and small intestinal (SI) LP (g) of GF mice that were colonized with B. fragilis WT9343 strain or ΔΔ9343 strain for 14 days. (h) ELISA quantification of B. fragilis 9343-specific IgA in fecal samples of GF mice that were colonized with B. fragilis WT9343 strain or ΔΔ9343 strain for 14 days. Error bars represent mean ± SEM. ns, not significant. Statistical comparisons were performed using two-tailed unpaired Student’s t test (a, e, h), two-way ANOVA with Sidak’s multiple comparisons test (d, f, g). n = 3 (d), n = 5 (e-h) independent biological samples.
