Extended Data Fig. 8: Early life IgA Coating Bacteria.
From: Bacterial and host fucosylation maintain IgA homeostasis to limit intestinal inflammation in mice
Early life IgA Coating Bacteria Facilitates the Activation of GC B Cells in PPs. (a) The number of GL-7+IgD− GC B cells in PPs of IgA-/- mice after antibiotic treatment. (b) Representative plots of flow cytometry and the frequency of activated B220+ GL-7+IgD− GC B cells in PPs of WT and IgA-/- mice at the age of 3-4 weeks and 20-24 weeks. (c) Representative plots of flow cytometry and the frequency of activated B220+ GL-7+IgD− GC B cells in PPs of WT and IgA-/- mice. 5-month-old IgA-/- mice were fed with WT microbiota in drinking water for 3 weeks. (d-e) Representative flow cytometry and the frequency of GL-7+IgD− GC B cells (B) and CD138+IgA+ B cells (C) in PPs of WT and pIgR-/- mice. pIgR-/- mice were treated with antibiotics and colonized with BFWT9343 or BFΔΔ9343 for 2 weeks. The data are representative of two independent experiments (a, d, e). Error bars represent mean ± SEM. ns, not significant. Statistical comparisons were performed using two-tailed unpaired Student’s t test (a, c, d, e); two-way ANOVA with Sidak’s multiple comparisons test (b). n = 3 (c), n = 4 (b), n = 5 (a, d, e) independent biological samples.
