Extended Data Fig. 6: tetA mutants obtained from LySE evolution for tigecycline resistance. | Nature Microbiology

Extended Data Fig. 6: tetA mutants obtained from LySE evolution for tigecycline resistance.

From: Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system

Extended Data Fig. 6: tetA mutants obtained from LySE evolution for tigecycline resistance.The alternative text for this image may have been generated using AI.

a. Screening of 32 clones from LySE E5 evolution of tetA gene for tigecycline resistance. b. Sanger sequencing of the tetA gene in the 32 evolved clones. Convergence of sequences in the promoter region (pCAT) as well as in the coding sequence (V145A, G283S). c. Normalized tetA expression levels in E5 LySE compared to E1 pools quantified by RT-qPCR. Data shown as mean ± SD of n = 3 independent experiments.

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