Extended Data Fig. 7: LySE evolution of ethylene glycol (EG) assimilation pathway and selection with EG as the sole carbon source. | Nature Microbiology

Extended Data Fig. 7: LySE evolution of ethylene glycol (EG) assimilation pathway and selection with EG as the sole carbon source.

From: Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system

Extended Data Fig. 7: LySE evolution of ethylene glycol (EG) assimilation pathway and selection with EG as the sole carbon source.The alternative text for this image may have been generated using AI.

Growth rate analysis of E. coli MG1655 containing the EG assimilation pathway phagemid following four rounds (E1 to E4) of LySE selection with EG as the sole carbon source. Initial LySE evolution showed no improvements, likely due to combined mutagenesis and carbon restriction stresses causing poor growth and loss of potential mutants. We therefore implemented a semi-relaxing selection protocol with initial glucose supplementation followed by gradual glucose withdrawal (Extended Data Fig. 11). Blue lines (MG1655 + pAN29) represent the growth curves of cells containing the EG assimilation pathway phagemid. Red lines (Control) show the negative control which is E. coli MG1655 without the phagemid. Cultures were grown in M9 minimal medium with 10 g/L EG. Data shown as mean ± SD of n = 3 independent experiments. The best replicate from each round was picked for subsequent rounds of LySE.

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