Extended Data Fig. 9: Characterization of phagemid pools obtained from LySE evolution versus ALE evolution for EG assimilation. | Nature Microbiology

Extended Data Fig. 9: Characterization of phagemid pools obtained from LySE evolution versus ALE evolution for EG assimilation.

From: Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system

Extended Data Fig. 9: Characterization of phagemid pools obtained from LySE evolution versus ALE evolution for EG assimilation.The alternative text for this image may have been generated using AI.

a. Growth curves of E. coli strains containing EG assimilation phagemids evolved by five generations of LySE (LySE E5), evolved by five generations of ALE (ALE E5), and the unevolved E. coli strain containing the wild-type EG assimilation phagemid (WT). We observed improved growth for both evolved strains compared to the wild type, and a greater improvement for the LySE evolved strain. Data shown as mean ± SD of n = 3 independent experiments. b. Nanopore sequencing of the EG assimilation phagemid in 32 ALE-evolved clones detected no mutations, indicating that improved growth is likely attributed to genomic mutations. c. Nanopore sequencing of the EG assimilation phagemid in 28 LySE-evolved clones detected mutations throughout the phagemids.

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