Extended Data Fig. 10: Characterization of eight isolated phagemids evolved by LySE for EG assimilation. | Nature Microbiology

Extended Data Fig. 10: Characterization of eight isolated phagemids evolved by LySE for EG assimilation.

From: Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system

Extended Data Fig. 10: Characterization of eight isolated phagemids evolved by LySE for EG assimilation.The alternative text for this image may have been generated using AI.

a. Growth curves of E. coli bearing eight isolated evolved phagemids (EGA1-8) after five generations of LySE, and wild-type phagemid (WT) in M9 media with 10 g/L EG and no glucose. b. Endpoint biomass (t = 48 h) of the growth curves. Five clones exhibited accelerated growth on EG compared to the wild type (Starting from best: EGA5, EGA3, EGA7, EGA8, EGA4). Data are the mean ± standard deviation from three (n = 3) independent biological replicates. Significance was tested via two-tailed unpaired two-sample t-test between each respective mutant and the wild-type variant. EGA3: p = 2.29 × 10−5; EGA4: p = 8.21 × 10−3; EGA5: p = 2.53 × 10−5; EGA7: p = 3.26 × 10−4; EGA8: p = 1.35 × 10−4. * p < 0.01; ** p < 0.001; *** p < 0.0001.

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