Extended Data Fig. 4: Mutational rates and spectra of LySE T7 DNAP variants. | Nature Microbiology

Extended Data Fig. 4: Mutational rates and spectra of LySE T7 DNAP variants.

From: Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system

Extended Data Fig. 4: Mutational rates and spectra of LySE T7 DNAP variants.The alternative text for this image may have been generated using AI.

a. Mutational frequencies of engineered T7 DNAP variants (v1-v9) determined by Illumina NGS. Colours indicate specific transition and transversion types. ‘n.d.’ indicates not detected. b. Mutational spectra heatmaps displaying the frequency of each substitution type calculated as a percentage of the total number of mutations observed. Rows represent the reference base; columns represent the mutated base. The sum of all values in the matrix equals 100%. c. Distribution of Mutational spectra across a 39-kb BAC-phagemid. Top: Schematic of the DNA construct including T7 ori/pac, antibiotic selection markers (KanR/CmR), bacterial artificial chromosome (BAC), Yeast artificial chromosome (YAC), and yeast chromosomal DNA elements. Bottom: Mutational spectra calculated for consecutive 10,000 bp windows for variants v8 and v9.

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