Table 1 Effective library sizes of a LySE experiment

From: Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system

Selection vessel

Volume (ml)

Library size

Whole microplate

96-well plate

0.2

1.28 × 105

1.23 × 107

96-deepwell plate

1

6.40 × 105

6.14 × 107

Culture tube

5

3.20 × 106

 

250-ml flask

100

6.40 × 107

 

500-ml flask

200

1.28 × 108

 

2-l flask

800

5.12 × 108

 

5-l flask

2,000

1.28 × 109

 
  1. An example of a 10,000 bp phagemid is shown to represent a typical metabolic pathway, with 4 copies packaged per T7 bacteriophage evolved with T7 DNAP v9. The example assumes a LySE v9 packaging efficiency of 1.60 × 108, a transduction dilution factor of 100 and a selection dilution factor of 10, resulting in a phagemid concentration of 6.40 × 105 per ml cells. Expected library sizes in terms of the number of phagemids is estimated for each scale of selection.
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