Extended Data Table 1 MT1 radioligand affinity

From: Structural basis of ligand recognition at the human MT1 melatonin receptor

  1. Affinity was determined using radioligand competition binding and [3H]melatonin to yield Kd or Ki affinity estimates (number of independent experiments in square brackets) for wild-type (WT) and mutant receptors expressed in HEK293T cells. MT1-CC, crystal construct (including PGS fusion) expressed in Sf9 cells; MT1-9mut, construct combining all crystallogenic mutations, expressed in HEK293T cells. MT1–PGS, wild-type construct with PGS fusion. ND, not determined. Binding isotherms are shown in Supplementary Fig. 1. To determine the effect of NaCl, binding assays were performed in the presence of 147 mM NaCl (binding isotherms in Supplementary Fig. 2).
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