Extended Data Table 6 Functional data (Gi/o GloSensor) for mutants of the YPYP motif and the ligand binding site

From: Structural basis of ligand recognition at the human MT1 melatonin receptor

  1. Data were acquired with wild-type MT1 and mutants by using GloSensor to measure Gi/o-mediated cAMP inhibition via isoproterenol stimulation. Data represent means of EC50 (number of independent experiments in square brackets), shown as mean pEC50 ± s.e.m. as well as EMAX, shown as mean %EMAX ± s.e.m. %EMAX is relative to wild-type receptor in columns, and %EMAX* is relative to melatonin in rows. Mutant effects were calculated by the change in relative activity, or log(EMAX/EC50) subtracting wild-type from mutant activity. ND, not determined. Dose–response curves are shown in Supplementary Fig. 5.
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