Extended Data Fig. 1: Categories and coexpression network of genome-wide RNAi screening hits.
From: Two conserved epigenetic regulators prevent healthy ageing
a, Effects of representative positive clones on BAS-1 protein levels in Pbas-1::bas-1::gfp transgenic worms. White arrows indicate NSM neurons. Scale bar, 20 µm. Images are representative of three independent experiments. b, Effect of each screening hit on the expression of BAS-1::GFP. The GFP fluorescence in NSM neurons of worms individually treated with the 59 screening hits, also shown in Supplementary Table 1, was normalized to that in worms treated with the control RNAi. P values were determined by unpaired two-tailed t-test (see Supplementary Table 1 for numbers of tested worms and exact P values). c, Categories of genes corresponding to the 59 screening hits identified by RNAi. d, Coexpression network of screening hits and their partners. The network was constructed with GeneMANIA. Black and grey dots indicate screening hits and their partners, respectively. Blue and orange dots show screening hits whose human homologues are involved in age-related neurodegeneration and cell senescence, respectively. e, Pharyngeal pumping in TU3401 worms (which express a dsRNA channel, SID-1, in neurons) after RNAi treatment in the presence of 20 μM 2′-deoxy-5-fluorouridine (FUDR). The genes from the top 20 screening hits were examined. Numbers of tested worms are shown beneath the bars. Data shown are means ± s.e.m.; *P < 0.05; **P < 0.05; ***P < 0.001; ns, not significant (one-way ANOVA with Dunnett's test; see Supplementary Information for exact P values)
