Extended Data Fig. 2: BAZ-2 and SET-6 regulate age-related decline in BAS-1 expression.
From: Two conserved epigenetic regulators prevent healthy ageing
a, Expression patterns of BAZ-2 and SET-6. Scale bar, 30 μm. Images are representative of three independent experiments. b, Representative fluorescence images (left) and quantitative analysis (right) of BAZ-2 and SET-6 expression. We quantified only those cells whose nuclear morphology was clearly visualized with DAPI. Scale bar, 10 μm. n = 14 and 18 worms for BAZ-2 and SET-6, respectively. c, d, Representative western blots (c) and quantitative analysis (d) of age-related changes in endogenous SET-6 and BAZ-2 protein levels in genome-edited baz-2GFP::FLAG;set-6GFP::HA worms. n = 5 independent experiments. Tubulin expression is used as a reference. For gel source data, see Supplementary Fig. 2. In d, each data point represents the result of one independent experiment. e, Fluorescence images (left) and quantitative analysis (right) of BAS-1 expression in baz-2 and set-6 mutant worms. Quantitative analysis of BAS-1 levels was performed by measuring GFP fluorescence intensity in the soma of NSM neurons. White arrows indicate NSM neurons. Scale bar, 15 μm. The numbers of tested worms are shown beneath the bars. All data shown are means ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001 (d, Kruskal–Wallis test; e, one-way ANOVA with Dunnett’s test; see Supplementary Information for exact P values).
