Extended Data Fig. 2: Cryo-EM processing and 3D reconstruction workflow.
From: Structural basis of tethered agonism of the adhesion GPCRs ADGRD1 and ADGRF1
a–f, Results of ADGRD1–miniGs. a, Data processing workflow. b, Cryo-EM map coloured according to local resolution (in Å). c, Representative cryo-EM image from one independent experiment. d, Two-dimensional (2D) averages. e, Gold-standard Fourier shell correlation (FSC) curve showing an overall resolution of 2.8 Å. f, Cross-validation of model to cryo-EM density map. FSC curves for the final model versus the final map and half maps are shown in black, green and yellow, respectively. g–l, Results of ADGRF1–miniGs. g, Data processing workflow. h, Cryo-EM map coloured according to local resolution (in Å). i, Representative cryo-EM image from two independent experiments with similar results. j, 2D averages. k, Gold-standard FSC curve showing an overall resolution of 3.1 Å. l, Cross-validation of model to cryo-EM density map. m–r, Results of ADGRF1–miniGi1. m, Data processing workflow. n, Cryo-EM map coloured according to local resolution (in Å). o, Representative cryo-EM image from three independent experiments with similar results. p, 2D averages. q, Gold-standard FSC curve showing an overall resolution of 3.4 Å. r, Cross-validation of model to cryo-EM density map. s–x, Results of ADGRF1(H565A/T567A)–miniGi1. s, Data processing workflow. t, Cryo-EM map coloured according to local resolution (in Å). u, Representative cryo-EM image from two independent experiments with similar results. v, 2D averages. w, Gold-standard FSC curve showing an overall resolution of 3.0 Å. x, Cross-validation of model to cryo-EM density map.
