Extended Data Fig. 11: MYB directly regulates target gene expression, and CD62L⁺ T_PEX cells have a superior potential to give rise to CX3CR1⁺ T_EX cells.

(a–d) Representative tracks showing MYB ChIP–seq peaks in the LEF1 (a), E2F1 (b), GZMA (c), and MYB (d) gene loci of human Jurkat T cells and ATAC-seq peaks of T_PEX and T_EX cells in the corresponding mouse gene loci aligned according to the sequence conservation. (e–g) Congenically marked naive P14 cells were transferred to primary recipient mice (R1), which were subsequently infected with LCMV-Cl13. The indicated subsets of P14 cells were sorted at 28 dpi and re-transferred to naive secondary recipient mice (R2), which were then infected with LCMV-Armstrong. Splenic P14 T cells in R2 mice were analysed at 8 dpi. (e) Schematic of the experimental set-up. (f) Flow cytometry plots of progenies recovered at 8 dpi. (g) Cell numbers (left) and quantification of PD-1 expression (right) in P14 T cell populations derived from the indicated transferred subsets at 8 dpi. (h, i) Congenically marked naive P14 T cells were transferred into primary recipient mice (R1), which were then infected with LCMV-Docile. The indicated subsets of P14 T cells were sorted at 7 dpi and 7.5×10⁴ cells were re-transferred to infection-matched (LCMV-Docile) secondary recipient mice (R2). Splenic P14 T cells of R2 mice were analysed at day 28 post re-transfer. (h) Schematic of the experimental set-up. (i) Flow cytometry plots and box plots showing the frequencies of CX3CR1⁺ and CD101⁺ cells among recovered T_EX cells derived from the indicated re-transferred T_PEX subsets at day 28 post re-transfer. Data are representative of two independent experiments. P values are from Mann–Whitney tests (g) and two-tailed unpaired t-test (i); P > 0.05, not significant (n.s.).

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