Extended Data Fig. 4: Primary cohort recall upon heterologous HA protein boosting.
From: Molecular fate-mapping of serum antibody responses to repeat immunization
(a) Comparison of de novo FLAG+ antibody responses to HAFM1 in the presence or absence of primary infection with influenza virus PR8. PR8>FM1>FM1 data are for the same samples as in Fig. 3d, re-measured in the same assay as Ø>FM1>FM1. (b) Schematic representation of HA prime-boost strategy. S1pr2-IgkTag/Tag mice were primed i.p. with HANY95 in alhydrogel and boosted homologously or heterologously with HAN99 or HACA09 in alhydrogel as indicated. (c) Full time course of anti-HA tag-specific ELISA reactivity (optical density at 1:100 dilution) of the same mice shown in Fig. 3f. Anti-HANY95 (top), HANC99 (middle) and HACA09 (bottom) ELISAs are shown, with Strep (left) and FLAG (right) detection. (d) Relationship between primary addiction and antigenic distance for infection and immunization experiments. Graph compiles primary addiction indices from Fig. 3d and 3g. Bars are ordered by amino acid identity between the priming and boosting HAs. P-values are for one-way ANOVA, with % identity as a categorical variable, or Pearson correlation, with (100 – % identity) as a linear variable.
