Extended Data Fig. 9: Pharmacological blockade of adrenergic receptors reprograms tumor infiltrating T cells in PDAC.

a, Heatmap showing the top 10 marker genes for each T cell cluster. Expression is normalized across cells. b, Heatmap showing differentially regulated pathways in CD8⁺ T cells extracted from the PDAC scRNA Seq dataset. T cells were grouped by condition and z score of median pathway activity is shown. c, UMAP visualization of CD8⁺ T cells extracted from the PDAC scRNA Seq dataset. Cells were annotated using SingleR with the published exhaustion subsets from PRJNA497086. Stacked bar graphs show frequency of exhaustion subsets per treatment condition as annotated per PRJNA497086. d, Expression of the beta-blocker signature identified in Fig. 5l in human T cells isolated from PDAC patients under beta-blocker therapy (n = 2) vs PDAC patients without beta-blocker therapy (n = 15). Data from GSE155698. Tukey’s test. Boxplots show median. The lower and upper hinges correspond to the first and third quartiles. The upper whisker extends from the hinge to the largest value no further than 1.5 * IQR from the hinge. Two-sided statistical tests were used. **** indicates a p value < 0.0001, ***<0.001, **<0.01, *<0.05.