Extended Data Fig. 1: Mouse gastrulation is preceded by compartmentalised glucose activity.
From: Selective utilization of glucose metabolism guides mammalian gastrulation
(a-c) Visualizing glucose uptake in early-streak (a) and mid-to-late-streak (b, c) mouse gastrulas via live-imaging of 2-NBDG (fluorescent glucose analog). Illustrations demonstrate developmental stage and plane of imaging. 15 independent experiments. (a) 2-NBDG signal is high in the transitionary epiblast on the posterior side of the embryo distal to the primitive streak. (b, c) 2-NBDG remains high in the transitionary epiblast distal to the primitive streak (purple cells in illustration, wave 1), is low in the primitive streak itself (grey cells in illustration), and is high in the lateral mesoderm that has exited the primitive streak as mesenchyme (yellow cells in illustration). Scale bars, 20 µm.
