Extended Data Fig. 4: Stem cell models shows the direct impact of glucose metabolism on mesoderm fate acquisition.
From: Selective utilization of glucose metabolism guides mammalian gastrulation
(a) Representative images of mouse gastruloids treated with 2-DG and YZ9. 2-DG treated gastruloids fail to elongate and do not express T. YZ9 treated gastruloids elongate and have T expression comparable to control. (b) Quantitative RT-PCR demonstrates significantly reduced T and Etv4 in 2-DG treated gastruloids. Plots show mean + SD. Dunnett’s multiple comparisons test following one-way ANOVA. P-values shown. N = 3 replicates. (c) Representative images from mXEN cells treated with 2-DG+BrPA, Azaserine, YZ9, Galloflavin, and 6AN for 24 h and treated with Oligomycin for 6 h, all shown to maintain expression of endoderm marker GATA6 (red). N = 3 independent experimental replicates examined for each condition. (d) Representative images from live imaging of early-streak stage gastrulas incubated with dyes for active mitochondria (MitoTracker, top) and mitochondrial membrane potential (TMRM, bottom). Zoomed-in panels visualizing the epiblast-VE boundary. Heaptmap intensity colours via Fiji’s LUT. N ≥ 3 embryos examined for each dye.
