Supplementary Fig. 8: Additional PDAC-A replicates and cross-sample MIA map. | Nature Biotechnology

Supplementary Fig. 8: Additional PDAC-A replicates and cross-sample MIA map.

From: Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas

Supplementary Fig. 8: Additional PDAC-A replicates and cross-sample MIA map.The alternative text for this image may have been generated using AI.

(a) Tissue histology for PDAC-A-2 and PDAC-A-3 used for ST. Highlighted in red are the cancer cells in the tissue. Scale bar, 1 mm. 3 ST replicates were generated from this PDAC-A. (b) Distribution of UMIs across the PDAC-A-2 and PDAC-A-3 ST tissue. (c) ST data statistics for PDAC-A-2 and PDAC-A-3 samples. Top, histogram of UMIs per spot. Bottom, histogram of genes expressed per spot. (d) MIA map of the PDAC-A-2 tissue regions. Color bar above MIA map indicates the clustering assignments. The hypergeometric distribution was used to calculate p-values shown with 19,738 genes in the background. (e) Hypergeometric between gene sets specific to PDAC-A-1 and PDAC-A-2 tissue regions. Color bar on left corresponds to PDAC-A-1 ST clustering assignments, and color bar on top corresponds to PDAC-A-2 ST clustering assignments.

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