Supplementary Fig. 4: Estimating the total number of cells captured by an ST spot. | Nature Biotechnology

Supplementary Fig. 4: Estimating the total number of cells captured by an ST spot.

From: Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas

Supplementary Fig. 4: Estimating the total number of cells captured by an ST spot.The alternative text for this image may have been generated using AI.

ST spots were mapped back onto the H&E stained tissue, and brightfield images were extracted from the location of each ST spot. In each enlarged spot, the dark purple dots are nuclei, and the purple background is the cytoplasm and extracellular space. Enlarged spots shown demonstrate that ST spots can capture as few as ten or less cells and as many as a few dozen cells. Scale bar, 1 mm.

Back to article page