Extended Data Fig. 8: Characterization of host cells infiltrating MASTER.
From: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells
A) Timeline of experiment B) Representative FACS plot showing efficient engraftment of human PBMCs (Hu-CD45 + CD3+) in blood. C-D) Different subsets of mouse and human cells that infiltrated MASTER. Cells were gated on live cells. Data in d-e represent mean ± SEM and median of three biologically independent samples. E) Phenotype of the engrafted human T cells that infiltrated into the scaffold. Cells were gated on human-CD45 + CD3 + cells. Data represent mean ± SEM of four biologically independent samples F) FACS plot showing no GFP expression in cells infiltrating MASTER, in blood and in the skin surrounding the scaffold.
