Extended Data Fig. 6: Identification of components sequestered in EML4-Alk granules. | Nature Biotechnology

Extended Data Fig. 6: Identification of components sequestered in EML4-Alk granules.

From: Computationally designed sensors detect endogenous Ras activity and signaling effectors at subcellular resolution

Extended Data Fig. 6: Identification of components sequestered in EML4-Alk granules.The alternative text for this image may have been generated using AI.

a, (top) Representative epifluorescence images of Beas2B cells expressing GFP-tagged EML4-Alk v1 and immunostained for hits identified in the mass spectrometry analysis. Arrows indicate co-localization of EML4-Alk v1 with probed protein. (bottom) Colocalization analysis (n = 3 biologically independent experiments per condition). b, Representative immunoblot of Beas2B cells transfected with V5-tagged EML4-Alk v1 full length or with trimerization domain deleted (∆TD), subjected to V5 immunoprecipitation, and probed for hits identified in the MS analysis. Bar graphs represent mean ± s.e.m.

Back to article page