Supplementary Fig. 12: Synergism of MTX and (S)-JQ1.
From: MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation
a, Table of IC50 values for (S)-JQ1 and MTX as reported in the Welcome Trust-Genomics of Drug Sensitivity in Cancer database (WT; http://www.cancerrxgene.org), or produced in house (right two columns), for the different cell lines. b, Determination of IC50 values for (S)-JQ1 (red) and MTX (dark red) in the indicated cell lines. Three biological replicates were done for each experimental condition (mean ± SD). c, Dose response matrices displaying cell viability of HeLa and KBM7 treated for 72 h with (S)-JQ1 and MTX alone or in combination. Means from two biological replicates. d, Deviations from Bliss additivity for drug matrices from Fig. 4c and Supplementary Fig. 6c. e, Knock-down of MTHFD1 in A549 cells followed by 72 h treatment with increasing concentrations of (S)-JQ1. Mean ± SD from n = 2 independent samples. f, Induction of caspase activity in HAP1 WT and MTHFD1 knock-out cells treated with for 72 h with (S)-JQ1 or MTX. Mean ± SD from n = 2 independent samples.
