Extended Data Fig. 8: Clustering dynamics of PcG-associated condensates.
a Quantification of the number and the area of the light-induced droplets of BMI-Cry2 at different time points. First panel shows the distribution changes in the time window of 300 seconds post-stimulation. Second panel shows the formation and disassembly of BMI-Cry2 clusters within a time window of 35 minutes. b Quantification of the relative frequency of small (<0.1μm2) and large (>0.1μm2) droplets of BMI-Cry2 at different time points. c Representative images of dual immunostaining for BMI1 and RING1B (upper panels) or H3K27me3 (middle panels) or BRD4 (lower panels) in WT MSCs; scale bar, 10 µm. Pearson coefficient was represented as box plots, indicating the median (middle line), the first and third quartiles (box), and the 10th and 90th percentile (error bars). The number of analyzed nuclei is reported in figure as n. d Representative images of immunostaining for H3K27me3 and RING1B in NIH3T3 expressing BMI-Cry2, after light-induced clustering, Scale bar, 10 μm. Pearson Coefficient between BMI-Cry2 and H3K27me3 or RING1B was represented as box plots indicating the median (middle line), the first and third quartiles (box), and the 10th and 90th percentile (error bars). The number of analyzed nuclei is reported in figure as n.
