Fig. 1: Neutralization of spike protein pseudotyped lentiviruses.
From: A potent and broad neutralization of SARS-CoV-2 variants of concern by DARPins
a, Illustration of the engineered monomeric and trimeric DARPin molecules and their neutralization of pseudotyped lentiviruses. B.1.1.529* harbors a chimeric spike protein in which the RBD region (residue, 338–514) of B.1.351 was replaced with that from B.1.1.529 (BA.1). Residues in red were randomized during DARPin engineering. Data points are shown as mean ± s.d. from at least two independent experiments. b, SDS–PAGE gel analysis of the DARPin molecules under nonreducing and reducing conditions. The gel image was representative of three independent experiments. c, Size-exclusion chromatography of DARPin molecules under nonreducing conditions. The chromatograph was representative of two experiments.
