Extended Data Fig. 5: Engineering and characterization of ChemoX-ATP sensors.
From: A general method for the development of multicolor biosensors with large dynamic ranges
a. FRET/eGFP ratios of ATP sensors differing in the number of interface mutations (Supplementary Table S7) in presence (+ATP) or absence (+ATP) of 10 mM ATP. Asterisk indicates construct corresponding to the final sensor ChemoG-ATP. Shown are the means ±s.d. n = 3 technical replicates. b. Maximal FRET/eGFP ratio changes (MaxΔR/R0) of ATP sensors differing in the number of interface mutations (Supplementary Table S7). Asterisk indicates construct corresponding to the final sensor ChemoG-ATP. Shown are the means ±s.d. n = 3 technical replicates. c. Titrations of ChemoG-ATPSiR with ATP and structurally related molecules. d. Titrations of ChemoG-ATPSiR and ATeam 1.03 with ATP at different temperatures. e. Titrations of ChemoG-ATPSiR and ATeam 1.03 with ATP at different pH. Shown are the means ±s.d. of 3 technical replicates. f, g. Fluorescence intensity (FI) emission spectra of ChemoB-ATP (f) and ChemoR-ATP (g) labeled with SiR (Supplementary Table S7) in presence or absence of 10 mM ATP. Shown are the means of 3 technical replicates.
