Extended Data Fig. 2: Enrichment of HDAC proteins and their complexes in HEK293T Flp-In T-REx cells with SAHA-CA probe.
From: Profiling the interactome of oligonucleotide drugs by proximity biotinylation
a) SILAC-MS/MS for the competition experiment 200 nM SAHA-CA against 200 nM SAHA-CA + 1 mM valproic acid. The enrichment with SAHA-CA over Amide-CA is displayed on the x-axis and the depletion upon addition of valproic acid is displayed on the y-axis (SAHA-CA+VA/ SAHA-CA). b) Same as panel a but under soft wash conditions. c) Enrichment of HDAC proteins and their previously established complex partners in SILAC-MS/MS experiment with 200 nM SAHA-CA under harsh wash conditions. Only protein groups quantified in at least one replicate are shown. Statistically significant changes (p < 0.01) in enrichment are highlighted in bold. Replicate 2 corresponds to the enrichment plot in panel a. d) Enrichment of HDAC proteins and their previously established complex partners in SILAC-MS/MS experiment with 200 nM SAHA-CA under soft wash conditions. Only protein groups quantified in at least one replicate are shown. Statistically significant changes (p < 0.01) in enrichment are highlighted in bold. Replicate 1 corresponds to the enrichment plot in panel b.
