Supplementary Figure 3: Rfx7 deficiency affects NK cells in various tissues.

(a) The abundance of Rfx7 mRNA was determined by qRT-PCR in the indicated subsets of sorted NK cells from BM or spleen (SP) of wild type mice (relative to Polr2a). (b) A representative flow cytometric plot of NK cells (gated as NK1.1+CD3–CD19–) in the blood and liver of Ncr Rfx7wt/wt, and Ncr Rfx7fl/fl mice is shown (gated on CD45+ lymphocytes). Results represent the mean ± SD of n = 3 technical replicates (a) or the mean ± SEM of n = 3 mice/group (b) and are representative of at least two independent experiments (a,b). (b) Statistical comparison between the experimental condition lacking Rfx7 and control were performed; *p ≤ 0.05; Student’s t-test