Extended Data Fig. 4: In vivo efficacy of AUTO3 in Nalm6 NSG models. | Nature Medicine

Extended Data Fig. 4: In vivo efficacy of AUTO3 in Nalm6 NSG models.

From: CAR T cells with dual targeting of CD19 and CD22 in pediatric and young adult patients with relapsed or refractory B cell acute lymphoblastic leukemia: a phase 1 trial

Extended Data Fig. 4: In vivo efficacy of AUTO3 in Nalm6 NSG models.The alternative text for this image may have been generated using AI.

In vivo efficacy of AUTO3 in Nalm6 NSG models. (a-c) CD19+ CD22+ Nalm6 cells transduced to express luciferase were IV administered into NSG mice. After 4 days, 1×106, 3×106 or 5×106 transduced AUTO3 CAR T cells or matched number of non-transduced (NT) T cells were administered IV. a) Bioluminescent imaging (BLI) of the Nalm6 tumor burden measured post CAR T-cell injection. b) The mean total flux radiance quantified pre (day -1) and post administration of 1×106 and 3×106 CAR T-cells (n = 5 for FMC63 and AUTO3, n = 3 for NT). c) BLI of a CD19 knockout (CD19KO) Nalm6 NSG model. NSG mice were IV injected with luciferase positive CD19KO Nalm6 cells 8 days prior to the IV administration of 5×106 transduced CAR T-cells or matched number of non-transduced (NT) T cells (n = 5 biologically independent animals). Statistical analysis performed by two-way ANOVA with Bonferroni correction between FCM63 and AUTO3 over all time points. 1×106 dose **** P = 0.00002. 3×106 dose day 13 ****P < 0.00001, day 16 *** P = 0.00057.

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