Extended Data Fig. 3: Phasing results of two heterozygous variants in the same patient via IGV visualization and TA cloning sequencing.

a, IGV visualization of mapped reads containing c.631 T > C and c.630_631del detected in TUBB8 in case OE-2110. The two heterozygous variants located in different reads and were confirmed to be in trans. b, IGV visualization of mapped reads containing c.576 C > G and c.586 C > T detected in ASTL in case OE-3134. The two P/LP variants located in different reads and were confirmed to be in trans. c, IGV visualization of mapped reads containing c.289 G > C and c.374 T > C detected in PABPC1L in case OE-3036. The two P/LP variants located in different reads and were confirmed to be in trans. d, IGV visualization of mapped reads containing c.857 G > C and c.965 G > A detected in CDC20 in case OE-0182. The two P/LP variants located in different reads and were confirmed to be in trans. e, IGV visualization of mapped reads containing c.922 T > G and c.922_930del detected in ZFP36L2 in case OE-2089. The two P/LP variants located in different reads and were confirmed to be in trans. f, TA clone sequencing results of c.1506 A > T and c.1693 G > A in FBXO43 from case OE-3113. The two P/LP variants located in different clones and were confirmed to be in trans.