Extended Data Fig. 4: Validation of oMN and sMN population by comparing transcriptome profile with reference dataset.

(A-B) Heatmap shows differential expression levels of oMN- or sMN-specific genes in sorted HB9::GFP+ and PHOX2B::GFP+ of SOD1A4V and C9ORF72 ALS hiPSC lines (A), or reanalyzed mouse dataset from a previous literature (B). (C) Combined heatmap shows relative expression levels of oMN- or sMN-specific genes in sorted HB9::GFP+ and PHOX2B::GFP+ of SOD1A4V and C9ORF72 ALS hiPSC lines, and reanalyzed mouse dataset from a previous literature.