Extended Data Fig. 7: Common alteration of C21H26O3 in multiple ALS lines and direct comparision of altered metabolic metabolites by metabolomics analysis in isogenic control of SOD1A4V and SOD1A4V ALS hiPSC lines.

(A-B) Ion count values present commonly down-regulated C21H26O3 metabolic candidate in multiple ALS lines (A) and direct comparison of isogenic control of SOD1A4V and SOD1A4V lines (B) (*P<0.05, ***P<0.001, ****P<0.0001, n.s.: not significant, two-tailed; unpaired student’s t-test; p-values are indicated in each graph; at least n=3 for each lines; technical replicates; n=1 hESC control, n=3 hiPSC control, n=1 isogenic control hiPSC of SOD1A4V, n=6 C9ORF72, n=3 SOD1, n=3 TDP43, n=5 sporadic ALS hiPSC; biological replicates). (C-D) Heatmap shows the lists of selective metabolite candidate in isogenic control of SOD1A4V and SOD1A4V ALS hiPSC lines (n=6 for each group, n means 6 independent differentiation from 2 batch; technical replicates). Error bars: mean ± SEM.